Microbial Linear Plasmids

Author: Friedhelm Meinhardt
Publisher: Springer Science & Business Media
ISBN: 3540720251
Size: 26.25 MB
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A book that constitutes the first attempt to comprehensively assemble current knowledge of different types of such elements, highlight recent developments in the field, and challenge the distinction between viruses and linear plasmids. Linear plasmids of microbes represent a heterogenous group of extrachromosomal genetic elements initially assumed to be rare and peculiar. However, we now know that they are fairly frequently occurring plasmids in bacterial and eukaryotic species. Viral strategies to avoid shortening of the linear molecules during replication imply a common ancestry.

Microbial Megaplasmids

Author: Edward Schwartz
Publisher: Springer Science & Business Media
ISBN: 9783540854678
Size: 14.67 MB
Format: PDF, ePub, Mobi
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Megaplasmids are extrachromosomal genetic elements in the size range of 100 kb and larger. They are found in physiologically and phylogenetically diverse groups of bacteria and archaea. By definition, megaplasmids are not essential for the viability of their hosts under all growth conditions, but paradoxically many megaplasmids carry the genetic information for the defining and characteristic traits of the organism in which they reside. Microbial Megaplasmids reviews our knowledge of the extensively studied representatives, such as the catabolic plasmids of the pseudomonads, the rhizobial Sym plasmids, the Ti plasmids of the genus Agrobacterium and the giant enterobacterial virulence plasmids. It also presents snapshots of more recently discovered megaplasmids. The contribution of megaplasmids to the biology of their hosts is described, highlighting the interactions between megaplasmid and chromosomal genes.

E Coli Plasmid Vectors

Author: Nicola Casali
Publisher: Springer Science & Business Media
ISBN: 9781592594092
Size: 70.24 MB
Format: PDF, Kindle
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The authors present a comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, 1 vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids and the use of reporter genes, are also described.

Microbial Genome Methods

Author: Kenneth W. Adolph
Publisher: CRC Press
ISBN: 9780849344107
Size: 79.75 MB
Format: PDF, ePub, Mobi
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Learn to apply powerful molecular biology and genetics techniques to your microbial research with this easy-to-understand, practical guide. Microbial Genome Methods distills complex experimental procedures into fundamental techniques that can be readily adapted to your own research. In addition, this book provides background information specifically relevant to microbial research, as well as descriptions of expected results. The book is divided into two sections. Section one covers eukaryotic microbes, with emphasis on the yeast Saccharomyces cerevisiae. Section two covers prokaryotic microbes, focusing on Escherichia coli, and other important bacteria. These microbes were selected because they are widely studied and understood, but all information can be easily applied to the microbe you are investigating. Chapters are written by nationally and internationally recognized research scientists currently active in this field. Don't miss out on the tremendous boost these research techniques can give your work.

Microbial Protein Toxins

Author: Manfred Schmitt
Publisher: Springer Science & Business Media
ISBN: 9783540235620
Size: 18.48 MB
Format: PDF, Docs
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Since the initial establishment of Robert Koch’s postulates in the nineteenth century, microbial protein toxins have been recognized as a major factor of bacterial and fungal virulence. An increasing number of proteins produced and secreted by various bacteria, yeasts and plants are extremely toxic and most of them developed remarkably "intelligent" strategies to enter, to penetrate and to finally kill a eukaryotic target cell by modifying or blocking essential cellular components. This book describes the strategies employed by protein toxins to render their pro- and eukaryotic producers a selective growth advantage over competitors. In providing an up-to-date overview on the mode of protein toxin actions, it accommodates biomedically and biologically relevant toxin model systems. As a result, it significantly broadens our perspective on biochemical architecture and molecular ploy behind the lethal principles of pro- and eukaryotic toxins.

Veterinary Microbiology And Microbial Disease

Author: P. J. Quinn
Publisher: John Wiley & Sons
ISBN: 1118251164
Size: 57.64 MB
Format: PDF, Docs
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Microbiology is one of the core subjects for veterinary students, and since its first publication in 2002, Veterinary Microbiology and Microbial Disease has become an essential text for students of veterinary medicine. Fully revised and expanded, this new edition updates the subject for pre-clinical and clinical veterinary students in a comprehensive manner. Individual sections deal with bacteriology, mycology and virology. Written by an academic team with many years of teaching experience, the book provides concise descriptions of groups of microorganisms and the diseases which they cause. Microbial pathogens are discussed in separate chapters which provide information on the more important features of each microorganism and its role in the pathogenesis of diseases of animals. The international and public health significance of these pathogens are reviewed comprehensively. The final section is concerned with the host and is organized according to the body system affected. Tables, boxes and flow diagrams provide information in an easily assimilated format. This edition contains new chapters on molecular diagnostics and on infectious conditions of the skin, cardiovascular system, urinary tract and musculoskeletal system. Many new colour diagrams are incorporated into this edition and each chapter has been updated. Key features of this edition: Twelve new chapters included Numerous new illustrations Each chapter has been updated Completely re-designed in full colour Fulfils the needs of veterinary students and academics in veterinary microbiology Companion website with figures from the book as Powerpoints for viewing or downloading by chapter: www.wiley.com/go/quinn/veterinarymicrobiology Veterinary Microbiology and Microbial Disease remains indispensable for all those studying and teaching this essential component of the veterinary curriculum.